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Figure 3
Schematic overview of the grid-preparation workflow in the VitroJet. Using the control PC and the user interface, the operator is guided through the process. Durations for each stage of the workflow are indicative, depending on the selected settings. After selection of the deposition temperature and the decision on whether to perform vitrification, the system starts an initialization procedure. Subsequently, in the preparation phase the user is presented with instructions to prepare the system for processing up to 12 pre-clipped Autogrids. Once the preparation has finished, the protocol settings can be defined for each grid separately, and the instrument is able to automatically process Autogrids one by one. Once sufficient grids have been prepared, the operator is guided through a shutdown procedure to take out the grid boxes and finish the experiment.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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