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Figure 4
Subtomogram average of the mouse sperm doublet microtubule (DMT) and identification of CCDC105. (a) Cross-sectional view of the 48 nm repeat of the mouse sperm DMT determined using in situ cryo-ET and subtomogram averaging (EMDB entry EMD-41431; Chen, Shiozaki et al., 2023BB16). The overall resolution is estimated to be 7.7 Å. CCDC105, a microtubule inner protein of the B tubule, is shown in orange. (b) Longitudinal view of density corresponding to CCDC105 extracted from the DMT map. (c) An atomic model of CCDC105 showing its 16 nm periodicity. CCDC105 was identified using an unbiased search with an AlphaFold2 library containing 21 615 predicted models of the mouse proteome. CCDC105 has been confirmed at this location in other sperm DMTs using single-particle analysis cryo-EM (Leung et al., 2023BB54; Zhou et al., 2023BB92). (d) AlphaFold2 model of CCDC105.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 80| Part 4| April 2024| Pages 220-231
https://doi.org/10.1107/S2059798324001815
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