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Figure 5
Nuclease activity assay of SaTatD. (a) A metal-dependent DNase assay performed with 10 µM protein and 4 mM EDTA or 2 mM bivalent metals with 200 nM SaTatD genomic DNA (771 bp) as a substrate for 1 h at 37°C. Control groups (Con.) were prepared without SaTatD protein. (b) Protein or metal concentration-dependent DNase assay carried out with 2 mM Mg2+ or 4 µM protein, respectively, in the presence of 100 nM substrate for 1 h at 37°C. (c) Metal-dependent RNase assay. Fluorescence values (relative fluorescence units; RFU) were measured at Ex/Em = 490/520 nm for 1 h at 37°C. (d) DNase activity assay for the mutational study of SaTatD carried out with each mutant protein at 4 µM, 2 mM Mg2+, 200 nM SaTatD genomic DNA for 1 h at 37°C.

IUCrJ
Volume 7| Part 3| May 2020| Pages 509-521
ISSN: 2052-2525
https://doi.org/10.1107/S2052252520003917