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Structure of mouse ADP-ribosylhydrolase 3 (mARH3)

The crystal structure of ADP-ribosylhydrolase 3 from M. musculus has been determined and refined to a resolution of 1.8 Å. A detailed comparison with the human orthologue at the protein-sequence level as well as of the three-dimensional architecture is presented.

A novel acetate-bound complex of human carbonic anhydrase II

A complex of human carbonic anhydrase II with its inhibitor acetate is described where the ligand is found to bind in an orientation different from that previously described.

Purification, crystallization and preliminary X-ray diffraction analysis of aspartate semi­aldehyde de­hydrogenase (Rv3708c) from Mycobacterium tuberculosis

The enzyme aspartate semialdehyde dehydrogenase from M. tuberculosis has been expressed, purified and crystallized in two different crystal forms.

Preparation, crystallization and preliminary X-ray diffraction analysis of the DNA-binding domain of the Ets transcription factor in complex with target DNA

The complex between the Ets domain of Ets2 and its target DNA has been crystallized. The crystals diffracted to 3.0 Å resolution.

Crystallization and preliminary X-ray analysis of human Brn-5 transcription factor in complex with DNA

The human Brn-5 transcription factor has been crystallized in complex with DNA. Diffraction data were collected to 2.80 Å.

Crystallization and preliminary X-ray diffraction analysis of full-length and proteolytically activated pyruvate oxidase from Escherichia coli

The peripheral membrane flavoprotein pyruvate oxidase from E. coli has been crystallized in the full-length form and as a proteolytically activated truncation variant lacking the last 23 amino acids at the C-terminus.

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of variants of mono­amine oxidase from Aspergillus niger

Crystals of A. niger monoamine oxidase variants display P2₁ or P4₁2₁2/P4₃2₁2 symmetry, with eight or two molecules in the asymmetric unit, respectively.

Crystallization and preliminary X-ray studies of ferredoxin-NAD(P)⁺ reductase from Chlorobium tepidum

Ferredoxin-NAD(P)⁺ reductase from C. tepidum has been overexpressed in E. coli, purified and crystallized. Diffraction data were collected to 2.4 Å resolution.

Crystallization and preliminary X-ray diffraction analysis of vaccinia virus H1L phosphatase

A catalytically inactive mutant of the dual-specificity phosphatase H1L from vaccinia virus was expressed recombinantly, purified and crystallized by the microbatch method. The crystals belong to the tetragonal space group P422 and diffraction data were collected to 2.1 Å resolution using a synchrotron-radiation source. Attempts to derivatize these crystals with xenon gas lead to a space-group change to I422 with a smaller unit cell and a diffraction limit of 3.0 Å.

Crystallization and preliminary X-ray crystallographic characterization of TrmFO, a folate-dependent tRNA methyltransferase from Thermotoga maritima

T. maritima TrmFO was overexpressed, purified and crystallized. A diffraction data set was collected to a resolution of 2.6 Å.

Crystallization and preliminary X-ray analysis of RsbS from Moorella thermo­acetica at 2.5 Å resolution

Crystallization and selenium substructure solution of RsbS from Moorella thermoacetica, the first ab initio phased crystal structure from Diamond.

Expression, purification, crystallization and preliminary X-ray diffraction analysis of grass carp β₂-microglobulin

Grass carp β₂-microglobulin was expressed in E. coli, purified to homogeneity and crystallized using the hanging-drop vapour-diffusion method with PEG 2K as precipitant. The crystals obtained belong to the orthorhombic space group P2₁2₁2₁, with unit-cell parameters a = 38.72, b = 40.65, c = 71.12 Å.

Purification, crystallization and preliminary X-ray analysis of the peptidoglycan N-acetylglucosamine de­acetylase BC1960 from Bacillus cereus in the presence of its substrate (GlcNAc)₆

The peptidoglycan N-acetylglucosamine (GlcNAc) deacetylase BC1960 from B. cereus was crystallized in the presence of the substrate (GlcNAc)₆. The crystals belonged to space group P4₁2₁2 and diffracted to 2.38 Å resolution.

The purification, crystallization and preliminary X-ray diffraction analysis of di­hydrodipicolinate synthase from Clostridium botulinum

Dihydrodipicolinate synthase (DHDPS), an enzyme in the lysine-biosynthetic pathway, is a promising target for antibiotic development against pathogenic bacteria. Here, the expression, purification, crystallization and preliminary diffraction analysis of DHDPS from C. botulinum are reported.

Crystallization and preliminary X-ray study of native and selenome­thionyl β-1,4-mannanase AaManA from Ali­cyclobacillus acidocaldarius Tc-12-31

A. acidocaldarius Tc-12-31 β-1,4-mannanase was expressed in E. coli and purified. Crystallization and preliminarily X-ray crystallographic studies were performed for both the native mannanase and its selenomethionyl derivative.

Expression, purification, crystallization and preliminary X-ray characterization of two crystal forms of stationary-phase survival E protein from Campylobacter jejuni

Survival E (SurE) protein from Campylobacter jejuni, a Gram-negative mesophile, has been overexpressed in Escherichia coli as a soluble protein, successfully purified and crystallized in two distinct crystal forms.

Crystallization and preliminary X-ray diffraction studies of polyketide synthase-1 (PKS-1) from Cannabis sativa

Polyketide synthase-1 from C. sativa has been crystallized. The crystal diffracted to 1.55 Å resolution with sufficient quality for further structure determination.

Overproduction, purification and crystallization of a chondroitin sulfate A-binding DBL domain from a Plasmodium falciparum var2csa-encoded PfEMP1 protein

A chondroitin sulfate A-binding DBL important in placental malaria has been overproduced, purified and crystallized. Diffraction data were collected to 1.9 Å resolution.

Expression, purification, crystallization and preliminary X-ray analysis of the polysaccharide lyase RB5312 from the marine planctomycete Rhodopirellula baltica

This study describes the crystallization and preliminary X-ray analysis of the family PL1 polysaccharide lyase RB5312 from the marine bacterium R. baltica. Purified recombinant protein was crystallized; the crystals belonged to space group P2₁2₁2₁ and diffracted X-rays to a resolution of 1.8 Å.

Crystallization and preliminary X-ray crystallographic analysis of rabbit L-gulonate 3-de­hydrogenase

The preliminary X-ray crystallographic study of rabbit L-gulonate 3-dehydrogenase is described.